Recombinant Mouse TNFR1/TNFRSF1A Protein (His Tag)
PKSM040648
| Size: | Price: | $732 | |
| Qty: |
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Lead Time: 7~10 days
Product Details
| Catalog number | PKSM040648 |
| Expression_host | HEK293 Cells |
| Sequence | Met 1-Ala 212 |
| Bio_Activity | 1. Immobilized human TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 μg/mL. 2. Immobilized mouse TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 μg/mL. 3. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-1. 5 μg/mL in the presence of 0.1 ng/mL recombinant mouse TNFα. |
| Application | Functional ELISA |
| Accession | NP_ 035739.2 |
| Synonyms | CD120a;FPF;p55;p55-R;TNF-alphaR1;TNF-R;TNF-R-I;TNF-R1;TNF-R55;TNFalpha-R1;TNFAR;Tnfr-2;Tnfr1;TNFR60;TNFRI;TNFRp55 |
| Tag | C-His |
| Purity | > 96 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
