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SARS-CoV-2 Spike Protein IgM ELISA Kit

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Qty:
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Price:   $500
Cat Number:  E-EL-E603
Lead Time:   7~10 days

Reactivity:  Human

Assaytype:  Indirect-ELISA

Assaytime:  1.5h

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Properties

Format 24T, 96T, 96T*5, 96T*10
Assay time 1.5h
Sample type Human serum or plasma
Sample volume 10μL
Storage 2-8℃
Interpretation Qualitative
Application This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgM in serum or plasma.
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms COVID 19 IgM,S protein IgM

Test Principle

This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgM exists in the tested samples or not.

Assay Procedures

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