TMPRSS2 Polyclonal Antibody
Summary of Verified Samples
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WB
IHC
Western blot analysis of extracts of 22Rv1 cells using TMPRSS2 Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human stomach cancer using TMPRSS2 Polyclonal Antibody at dilution of 1:200 (40x lens).
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| Price: | $129 |
Datasheet
Test Application
Verified Samples
| Reactivity | Application |
| Human | WB (22Rv1,)  Western blot analysis of extracts of 22Rv1 cells using TMPRSS2 Polyclonal Antibody at dilution of 1:2000. (stomach cancer,)  Immunohistochemistry of paraffin-embedded Human stomach cancer using TMPRSS2 Polyclonal Antibody at dilution of 1:200 (40x lens). |
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Related Reagents
| Application | Products |
| WB | Western Blot Detection Kit(E-IR-R304) |
| IHC | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)(E-IR-R217) |
Product Details
| Isotype | IgG |
| Concentration | 1mg/mL |
| Storage | Store at -20°C. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Neuroscience |
| Conjugation | Unconjugated |
| Cellular cellular_localization | Cell membrane and Secreted. Activated by cleavage and secreted. |
| Tissue specificity | Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland. |
Immunogen Details
| Immunogen | Recombinant fusion protein of human TMPRSS2 (NP_001128571.1). |
| Abbre | TMPRSS2 |
| Synonyms | TMPRSS2,PP9284,PRSS10 |
| Swissprot | O15393 |
| Gene ID | 7113 |
| Calculated MW | 53kDa/57kDa |
| Observed MW | 52kDa |
Background
| This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |