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Western Blot Detection Kit

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Qty:
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Price:   $180
Cat Number:  E-IR-R304A
Lead Time:   7~10 days

Application:  WB

Storage:  RT/ 2~8°C/ -20°C

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Western Blot Kit (E-IR-R304)

Western Blot (WB) is the most common and classical experimental method for detecting protein expression in the biological science. However, due to differences in reagent sources, methods of operation and experience used by different Laboratory Technician, a large number of WB experimental results do not meet the requirement of paper publishing. Elabscience has been focusing on the field of immunology for many years and has accumulated a lot of experience in WB operation. In order to help researchers solve the above problems, the Western Blot kit is now available. The reagents in this kit are provided for classical WB experiments, including all reagents needed from protein extraction to result detection, which have advantages of simple operation, high detection sensitivity, low background and strong system stability. And Elabscience provides detailed operation description and professional technical support. Additionally, it offers you convenience and guarantee to work out ideal Western Blot experiment results.

 

As the PVDF membranes have two pore sizes for different molecular weights of target proteins respectively. In order to meet different experimental requirements, we have designed two specifications of Western Blot kits.

E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.

E-IR-R304B (PVDF membrane, 0.22 μm) is recommended for the proteins detection with molecular weights less than 20 kDa.

 

  • E-IR-R304A

E-IR-R304A (PVDF membrane, 0.45 μm) is recommended for the proteins detection with molecular weights more than 20 kDa.

Provided product

Product Catalog No Specification Storage Shelf life
RIPA Lysis Buffer (with PMSF and Na3VO4) E-BC-R327 5 mL/50 μL+50 μL -20℃ 6 months
Total Protein Colorimetric Assay Kit (BCA method) E-BC-K318 48 T 4℃/-20℃ 6 months
Excellent Chemiluminescent Substrate Detection Kit E-BC-R347 15 mL/15 mL 4℃ 6 months
Pre-stained Protein Marker (10-180 kDa) E-BC-R273 25 µL -20℃ 12 months
Goat Anti-Rabbit IgG(H+L)-HRP E-AB-1003 20 µL -20℃ 12 months
Skim Milk Powder E-BC-R337 15 g RT 6 months
PBS Buffer, pH7.4 (10 ×) E-BC-R187 100 mL RT 6 months
5 × SDS Loading Buffer E-BC-R288 1 mL × 2 -20℃ 6 months
Electrophoresis Buffer (10 ×) E-BC-R331 125 mL × 2 RT 6 months
Transmembrane Buffer (10 ×) E-BC-R333 125 mL × 2 4℃ 6 months
TBST Buffer (10 ×) E-BC-R335 125 mL × 2 RT 6 months
PVDF Membrane (0.45 μm, 8.5 × 6 cm) E-BC-R266 5 pieces RT 12 months

 

Alternative Reagent offer

Product Catalog No Specification Storage Shelf life
Goat Anti-Mouse IgG(H+L)-HRP E-AB-1001 20 µL -20℃ 12 months

 

Self-Prepared Reagent:Methanol

 

Instructions for user

This kit is for five gels (about 50 samples) with the Western Blot experiment.

Please store the reagents according to the storage conditions provided in the manual.

If you have any question about the product, please feel free to contact us or leave a message