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Origin and General Characteristics
||Calu-3 Cell Line
||Homo sapiens, Human
||Lung adenocarcinoma, derived from metastatic site: pleural effusion
||The patient had received prior therapy with cytoxan, bleomycin and adriamycin.
Culture Conditions and Handling
|Complete Growth Medium
||MEM +10% FBS +1% P/S
||Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
||Every 2 to 3 days
||Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
||Atmosphere: Air, 95%; CO2, 5%
Special Features of the Cell Line
||Yes, forms well differentiated grade I adenocarcinoma in nude mice.
||Antigen expression: Blood Type A; Rh＋
||Blood Type A; Rh＋
Recommendations for handling of cryopreserved cells